Supplemental Data

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 Tran Hoang, Wen- Tao Peng, Emmanuel Vanrobays, Nevan Krogan, Shawna Hiley, Ann L. Beyer, Yvonne N. Osheim, Jack Greenblatt, Timothy R.Hughes, and Denis L. J. Lafontaine, 2005

Esf2p, a U3-associated factor required for the initial steps of small ribosomal subunit synthesis. Mol Cell Biol. In the Press.

S1 Esf2p RNA partners, microarray analysis

S2 Esf2p is required for the efficient cycling of the box C+D snoRNA U14 and the box H+ACA snR3. See legend to FIG 5 for details.

S3 Protein and RNA composition of pre-rRNPs selected from Utp18p-TAP, in the presence and absence of Esf2p.

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Thiry, M., and D. L. J. Lafontaine, 2005

Birth of a nucleolus: the evolution of nucleolar compartments. Trends in Cell Biol. 15:194-199.

S1 : Fully referenced versions of Table 1 and 2

S2 : Nucleolar structure across evolution

Typical examples of plant (a), insect (b) and amphibian (c) nucleoli are illustrated.
     (a) meristematic cell from a pea cotyledonary bud. Pisum sativum.
     (b) Sf9 cell, Spodoptera frugiperda.
     (c) follicular cell from a North American triton, Pleurodeles waltlii. Matching ‘blueprint’ cartoons to the right. Key as in figure 1. Scale bars are 0.7 µm (panel a), 0.5 µm (panel b) and 0.4 µm (panel c), respectively.

S3 : Distribution of bi- and tripartite nucleoli across evolution

S4 and www.ulb.ac.be/sciences/rna/S4.pdf  : Length of rDNA transcription units versus intergenic regions across evolution

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 Lafontaine, D., and D. Tollervey, 1996

One-step PCR mediated strategy for the construction of conditionally expressed and epitope tagged yeast proteins. Nucleic Acids Res. 24:3469-71.

S1 Sequences of plasmids pTL26, pTL27, pTL28 and pTL32.
See also web.uni-frankfurt.de/fb15/mikro/euroscarf/data/