Lafontaine, D.L.J. , Riback,J.A., Bascetin, R., and Brangwynne, C.P. (2020) “The nucleolus as a multiphase liquid condensate” Nature Reviews Molecular Cell Biology |DOI:xxx In The Press

Leismann, J., Spagnuolo, M., Pradhan, M., Wacheul, L., Vu, M.A., Musheev, M., Mier, P., Andrade-Navarro, M.A., Graille, M., Niehrs, C., Lafontaine, D.L.J. , Roignant, J.-Y. (2020) “The 18S ribosomal RNA m6A methyltransferase Mettl5 is required for normal walking behavior in Drosophila” EMBO Reports e49443 |DOI:10.15252/embr.201949443 Supplemental Data

Heissenberger, C., Krammer, T.L., Rollins, J.A., Nagelreiter, F., Stocker, I., Wacheul, L., Shpylovyi, A., Snow, S., Grillari, J., Rogers, A., Lafontaine, D.L.J. , Schosserer, M. (2020) “The ribosomal RNA m5C methyltransferase NSUN-1 modulates healthspan and oogenesis in Caenorhabditis elegans” bioRxiv |DOI:10.1101/2020.03.16.993469 In Revision at eLIFE

Badrock, A.P., Uggenti, C., Wacheul, L., Crilly, S., Jenkinson, E.M., Rice, G.I., Kasher, P.R., Lafontaine, D.L.J.  Crow, Y.J., O’Keefe, R.T. (2020) “Analysis of U8 snoRNA Variants in Zebrafish Reveals how Bi-allelic Variants Cause Leukoencephalopathy with Calcifications and Cysts” The American Journal of Human Genetics 106: 694-706 |DOI:10.1016/j.ajhg.2020.04.003 Supplemental Data

Clerget G., Bourguignon-Igel V., Marmier-Gourrier N., Rolland N., Wacheul L., Manival X., Charron C., Kufel J., Méreau A., Senty-Ségault V., Tollervey D., Lafontaine D.L.J, Branlant C. and Rederstorff M. (2020) “Synergistic defects in pre-rRNA processing from mutations in the U3 snoRNA and U3-specific protein Rrp9″ Nucleic Acids Research 48(7): 3848-3868 |DOI:10.1093/nar/gkaa066 Supplemental data


Lafontaine, D.L.J. (2019) “Birth of nucleolar compartments: Phase separation-driven ribosomal RNA sorting and processing” Molecular Cell 76: 694-696 |DOI:10.1016/j.molcel.2019.11.015

Zhu, L., Richardson, T.M., Wacheul, L., Wei, M.-T., Feric, M., Whitney, G., Lafontaine, D.L.J. , Clifford P. Brangwynne (2019) “Controlling the material properties and rRNA processing function of the nucleolus using light” PNAS U.S.A. |DOI:10.1073/pnas/1903870116 Supplemental Data

Tran, N.V., Ernst, F.G.M., Hawley, B.R., Zorbas, C., Ulryck, N., Bohnsack, K.E., Bohnsack, M.T., Jaffrey, S.R., Graille, M. Lafontaine, D.L.J.  (2019) “The human 18S rRNA m6A methyltransferase METTL5 is stabilized by TRMT112″ Nucleic Acids Research |DOI:10.1093/nar/gkz619 nominated asNucleic Acids Research Breakthrough Article Supplemental Data

Roychowdhury, A., Joret, C., Bourgeois, G., Heurgué-Hamard, V., Lafontaine, D.L.J. , and Graille, M. (2019) “The DEAH-box RNA helicase Dhr1 contains a remarkable carboxyl terminal domain essential for small ribosomal subunit biogenesis” Nucleic Acids Research |DOI:10.1093/nar/gkz529 Supplemental Data

Catez, F., Dalla Venezia, N., Marcel, V., Zorbas, C., Lafontaine, D.L.J. , and Diaz, J.J. (2019) “Ribosome biogenesis: an emerging druggable pathway for cancer therapeutics” Biochemical Pharmacology |DOI:10.1016/j.bcp.2018.11.014


Marchand, V., Ayadi, L., Ernst, F.G., Hertler, J., Bourguignon-Igel, Valérie, Galvanin, A., Kotter, A., Helm, M. Lafontaine, D.L.J. , and Motorin, Y. (2018) “AlkAniline-Seq: profiling of m7G and m3C RNA modifications at single nucleotide resolution” Angewandte Chemie |DOI:10.1002/anie.201810946 Supplemental Data

Stamatopoulou, V., Parisot, P., De Vleeschouwer, C., Lafontaine, D.L.J. (2018) “Use of the iNo score to discriminate normal from altered nucleolar morphology, with applications in basic cell biology and potential in human disease diagnostics” Nature Protocols |DOI:10.1038/s41596-018-0044-3 Supplemental Data

Sharma, S., Hartmann, J.D., Watzinger, P., Klepper, A., Peifer, C., Koetter, P., Lafontaine, D.L.J., Entian, K.-D. (2018) “A single N1-methyladenosine on the large ribosomal subunit rRNA impacts locally its structure and the translation of key metabolic enzymes” Sci Rep 8: 11904 |DOI:10.1038/s41598-018-30383-z Supplemental Data

Jantsch, M. et al. (2018) “Positioning Europe for the EPITRANSCRIPTOMICS challenge” RNA Biol. |DOI:10.1080/15476286.2018.1460996

Pellegrino, S., Meyer, M., Zorbas, C., Boutcha, S.A., Saraf, K., Pelly, S.C., Yusupova, G., Evidente, A., Mathieu, V., Kornienko, A. Lafontaine, D.L.J., Yusupov, M. (2018) “The Amaryllidaceae alkaloid haemanthamine binds the eukaryotic ribosome to repress cancer cell growth” Structure 26: 1-10 |DOI:10.1016/j.str.2018.01.009 Supplemental Data

Vendramin, R., Verheyden, Y., Hideaki, I., Lucas, G., Nicolas, E., Saraf, K., Delli Ponti, R., Armaos, A., Izumikawa, K., Mestdagh, P. Lafontaine, D.L.J., Tartaglia, G. G., Takahashi, N., Marine, J.-C., Leucci, E. (2018) “SAMMSON fosters cancer cell fitness by enhancing concertedly mitochondrial and cytosolic translation” Nature Structural & Molecular Biology |DOI:10.1038/s41594-018-0143-4 Supplemental Data


Sharma, S., Marchand, V., Motorin, Y., Lafontaine, D.L.J. (2017) “Identification of sites of 2’-O-methylation vulnerability in human ribosomal RNAs by systematic mapping” Sci Rep 7: 11490 |DOI:10.1038/s41598-017-09734-9 Supplemental Data

Sharma, S., Yang, J., van Nues, R., Watzinger, P., Kötter, P., Lafontaine, D.L.J., Granneman, S., Entian, K.-D. (2017) “Specialized box C/D snoRNPs act as antisense guides to target RNA base acetylation” PLoS Genetics |DOI:10.1371/journal.pgen.1006804 Supplemental Data


Nicolas, E., Parisot, P., Pinto-Monteiro, C., de Walque, R., De Vleeschouwer, C., Lafontaine, D.L.J. (2016) “Involvement of human ribosomal proteins in nucleolar structure and p53-dependent nucleolar stress” Nature Communications |DOI:10.1038/ncomms11390 Supplemental Data

Langhendries, J.-L., Nicolas, E., Doumont, G., Goldman, S., Lafontaine, D.L.J. (2016) “The human box C/D snoRNAs U3 and U8 are required for pre-rRNA processing and tumorigenesis” Oncotarget |DOI:10.18632/Oncotarget.11148

Bar, D.Z., Charar, C., Dorfman, J., Yadid, T., Tafforeau, L., Lafontaine, D.L.J., Gruenbaum, Y. (2016) “Cell size and fat content of dietary restricted Caenorhabditis elegans are regulated by ATX-2, a novel mTOR repressor” PNAS U.S.A. |DOI:10.1073/pnas.1512156113

Leucci, E., Vendramin, R., Spinazzi, M., Laurette, P., Fiers, M., Wouters, J., Radaelli, E., Eyckerman, S., Leonelli,C., Vanderheyden,K., Rogiers,A., Hermans, E., Baatsen, P., Aerts, S., Amant, F., Van Aelst, S., van den Oord, J., de Strooper, B., Davidson, I., Lafontaine, D.L.J., Gevaert, K., Vandesompele, J., Mestdagh, P., Marine, J.-C. (2016) “Melanoma addiction to the long non-coding RNA SAMMSON” Nature 531: 518-522

Sobecki, M., Mrouj, K., Camasses, A., Parisis, N., Nicolas, E., Lleres, D., Gerbe, F., Prieto, S., Krasinska, L., David, A., Eguren, M., Birling, M.-C., Urbach, S., Hem, S., Déjardin, J., Malumbres, M., Jay, P., Dulic, V., Lafontaine, D.L.J., Feil, R., Fisher, D. “The cell proliferation antigen Ki-67 organises heterochromatin” Elife 2016 Mar 7;5. pii: e13722. |DOI:10.7554/eLife.13722.

Sloan, K.E., Warda, A.S., Sharma, S., Entian, K.-D., Lafontaine, D.L.J., Bohnsack, M. (2016) “Tuning the ribosome: the influence of rRNA modification on eukaryotic ribosome biogenesis and function” RNA Biology |DOI:10.1080/15476286.2016.1259781

Meyer, B., Wurm, J.P., Sharma, S., Immer, C., Pogoryelov, D., Kötter, P., Lafontaine, D.L.J., Wöhnert, J., Entian, K.-D. (2016) “Ribosome biogenesis factor Tsr3 is the aminocarboxypropyltransferase responsible for 18S rRNA hypermodification in yeast and humans” Nucleic Acids Research |DOI: 10.1093/nar/gkw244


Sharma, S., Lafontaine, D.L.J. “View From A Bridge: A New Perspective on Eukaryotic rRNA Base Modification” Trends in Biochemical Sciences Oct. 2015

Hauenschild, R., Tserovski, L., Schmid, K., Thüring, K., Winz, M.L., Sharma, S., Entian K.-D., Wacheul, L., Lafontaine, D.L.J., Anderson, J., Alfonzo, J., Hildebrandt, A., Jäschke, A., Motorin, Y., Helm, M. “The reverse transcription signature of N-1-methyladenosine in RNA-seq is sequence dependent” Nucleic Acids Res 2015. Sep 13. pii: gkv895. [Epub ahead of print]

Zorbas, C., Nicolas, E., Wacheul, L., Huvelle, E., Heurgué-Hamard, V., Lafontaine, D.L.J. (2015) “The human 18S rRNA base methyltransferases DIMT1L and WBSCR22-TRMT112 but not rRNA modification are required for ribosome biogenesis.” Molecular Biology of the Cell 26: 2080-2095.

Lafontaine, D.L.J. (2015). “Non-coding RNAs in ribosome synthesis and function.” Nature Structural & Molecular Biology 21(1): 11-19. Special Focus on ncRNAs. Editorial

Sharma, S., Langhendries, J.-L., Watzinger, P., Kötter, P., Entian, K.-D., Lafontaine, D.L.J. (2015) “Yeast Kre33 and Human NAT10 are conserved 18S rRNA cytosine acetyltransferases that modify tRNAs assisted by the adaptor Tan1/THUMPD1″. Nucleic Acids Res doi: 10.1093/nar/gkv075


Létoquart, J., Huvelle, E., Wacheul, L., Bourgeois, G., Zorbas, C., Graille, M., Heurgué-Hamard, V., Lafontaine, D.L.J. (2014). “Structural and functional studies of Bud23-Trm112 reveal 18S rRNA N7-G1575 methylation occurs on late 40S precursor ribosomes.” Proc Natl Acad Sci U.S.A. Dec 23;111(51):E5518-26. doi: 10.1073/pnas.1413089111. Epub 2014 Dec 8.

Stanicki, D., Boutry, S., Laurent, S., Wacheul, L., Nicolas, E., Crombez, D., Vander Elst, L., Lafontaine, D.L.J. and Muller, R. N. (2014). “Carboxy-silane coated iron oxide nanoparticles: a convenient platform for cellular and small animal imaging.” J Mater Chem B 2: 387-397. Last two authors are corresponding authors.

Ban, N., Beckmann, R., Cate, J. H., Dinman, J. D., Dragon, F., Ellis, S. R., Lafontaine, D.L.J., Lindahl, L., Liljas, A., Lipton, J. M., McAlear, M. A., Moore, P. B., Noller, H. F., Ortega, J., Panse, V. G., Ramakrishnan, V., Spahn, C. M., Steitz, T. A., Tchorzewski, M., Tollervey, D., Warren, A. J., Williamson, J. R., Wilson, D., Yonath, A. and Yusupov, M. (2014). “A new system for naming ribosomal proteins.” Curr Opin Struct Biol 24: 165-169.


Tafforeau, L., Zorbas, C., Langhendries, J. L., Mullineux, S. T., Stamatopoulou, V., Mullier, R., Wacheul, L. and Lafontaine, D.L.J. (2013). “The complexity of human ribosome biogenesis revealed by systematic nucleolar screening of Pre-rRNA processing factors.” Molecular Cell 51: 539-551. |DOI: 10.1016/j.molcel.2013.08.011 Supplemental Data


Schillewaert, S., Wacheul, L., Lhomme, F. and Lafontaine, D.L.J. (2012). “The evolutionarily conserved protein Las1 is required for pre-rRNA processing at both ends of ITS2.” Mol Cell Biol 32: 430-444.

Figaro, S., Wacheul, L., Schillewaert, S., Graille, M., Huvelle, E., Mongeard, R., Zorbas, C., Lafontaine, D.L.J. and Heurgue-Hamard, V. (2012). “Trm112 is required for Bud23-mediated methylation of the 18S rRNA at position G1575.” Mol Cell Biol 32: 2254-2267. Equal contribution of last two authors.

Mullineux, S. T. and Lafontaine, D. L. J. (2012). “Mapping the cleavage sites on mammalian pre-rRNAs: where do we stand?” Biochimie 94: 1521-1532.

Serviene, E., Luksa, J., Orentaite, I., Lafontaine, D.L.J. and Urbonavicius, J. (2012). “Screening the budding yeast genome reveals unique factors affecting K2 toxin susceptibility.” PLoS One 7: e50779. Equal contribution of last two authors.


Lamaye, F., Galliot, S., Alibardi, L., Lafontaine, D.L.J. and Thiry, M. (2011). “Nucleolar structure across evolution: the transition between bi- and tri-compartmentalized nucleoli lies within the class Reptilia.” J Struct Biol 174: 352-359.

Thiry, M., Lamaye, F. and Lafontaine, D.L.J. (2011). “The nucleolus: when 2 became 3.” Nucleus 2: 289-293.

Leporé, N. and Lafontaine, D.L.J. (2011). “A functional interface at the rDNA connects rRNA synthesis, pre-rRNA processing and nucleolar surveillance in budding yeast.” PLoS One 6: e24962.


Lafontaine, D.L.J. (2010). “A ‘garbage can’ for ribosomes: how eukaryotes degrade their ribosomes.” Trends Biochem Sci 35: 267-277.

Hernandez-Verdun, D., Roussel, P., Thiry, M., Sirri, V. and Lafontaine, D.L.J. (2010). “The nucleolus: structure/function relationship in RNA metabolism.” Wiley Interdiscip Rev RNA 1: 415-431.

Leporé, N. and Lafontaine, D.L.J. (2010). “[<< Catch me if you can >>: how the structural and functional integrity of eukaryotic RNA molecules is monitored by surveillance mechanisms].” Med Sci (Paris) 26: 259-266.


Wery, M., Ruidant, S., Schillewaert, S., Lepore, N. and Lafontaine, D.L.J. (2009). “The nuclear poly(A) polymerase and Exosome cofactor Trf5 is recruited cotranscriptionally to nucleolar surveillance.” RNA 15: 406-419.


Vanrobays, E., Leplus, A., Osheim, Y. N., Beyer, A. L., Wacheul, L. and Lafontaine, D.L.J. (2008). “TOR regulates the subcellular distribution of DIM2, a KH domain protein required for cotranscriptional ribosome assembly and pre-40S ribosome export.” RNA 14: 2061-2073.

Qiu, H., Eifert, J., Wacheul, L., Thiry, M., Berger, A. C., Jakovljevic, J., Woolford, J. L., Jr., Corbett, A. H., Lafontaine, D.L.J., Terns, R. M. and Terns, M. P. (2008). “Identification of genes that function in the biogenesis and localization of small nucleolar RNAs in Saccharomyces cerevisiae.” Mol Cell Biol 28: 3686-3699.


Chedin, S., Laferte, A., Hoang, T., Lafontaine, D.L.J., Riva, M. and Carles, C. (2007). “Is ribosome synthesis controlled by pol I transcription?” Cell Cycle 6: 11-15.


Thiry, M. and Lafontaine, D.L.J. (2005). “Birth of a nucleolus: the evolution of nucleolar compartments.” Trends Cell Biol 15: 194-199.

Hoang, T., Peng, W. T., Vanrobays, E., Krogan, N., Hiley, S., Beyer, A. L., Osheim, Y. N., Greenblatt, J., Hughes, T. R. and Lafontaine, D.L.J. (2005). “Esf2p, a U3-associated factor required for small-subunit processome assembly and compaction.” Mol Cell Biol 25: 5523-5534.


Vanrobays, E., Gelugne, J. P., Caizergues-Ferrer, M. and Lafontaine, D.L.J. (2004). “Dim2p, a KH-domain protein required for small ribosomal subunit synthesis.” RNA 10: 645-656.

Lafontaine, D.L.J. (2004). Eukaryotic ribosome synthesis. Protein Synthesis and Ribosome Structure. K. Nierhaus, Wiley-InterScience: 107-143.

Colau, G., Thiry, M., Leduc, V., Bordonne, R. and Lafontaine, D.L.J. (2004). “The small nucle(ol)ar RNA cap trimethyltransferase is required for ribosome synthesis and intact nucleolar morphology.” Mol Cell Biol 24: 7976-7986.


Verheggen, C., Lafontaine, D.L.J., Samarsky, D., Mouaikel, J., Blanchard, J. M., Bordonne, R. and Bertrand, E. (2002). “Mammalian and yeast U3 snoRNPs are matured in specific and related nuclear compartments.” EMBO J 21: 2736-2745.


Lafontaine, D.L.J. and Tollervey, D. (2001). “The function and synthesis of ribosomes.” Nature Reviews Mol Cell Biol 2: 514-520.

Verheggen, C., Mouaikel, J., Thiry, M., Blanchard, J. M., Tollervey, D., Bordonne, R., Lafontaine, D.L.J. and Bertrand, E. (2001). “Box C/D small nucleolar RNA trafficking involves small nucleolar RNP proteins, nucleolar factors and a novel nuclear domain.” EMBO J 20: 5480-5490. Equal contribution of last two authors.

Senger, B., Lafontaine, D.L.J., Graindorge, J. S., Gadal, O., Camasses, A., Sanni, A., Garnier, J. M., Breitenbach, M., Hurt, E. and Fasiolo, F. (2001). “The nucle(ol)ar Tif6p and Efl1p are required for a late cytoplasmic step of ribosome synthesis.” Molecular Cell 8: 1363-1373.

Lafontaine, D.L.J. and Tollervey, D. (2001). Ribosomal RNA. Encyclopedia of Life Sciences (, MacMillan – Nature

Dez, C., Henras, A., Faucon, B., Lafontaine, D.L.J., Caizergues-Ferrer, M. and Henry, Y. (2001). “Stable expression in yeast of the mature form of human telomerase RNA depends on its association with the box H/ACA small nucleolar RNP proteins Cbf5p, Nhp2p and Nop10p.” Nucleic Acids Res 29: 598-603.


Lafontaine, D.L.J. and Tollervey, D. (2000). “Synthesis and assembly of the box C+D small nucleolar RNPs.” Mol Cell Biol 20: 2650-2659.

Kufel, J., Allmang, C., Chanfreau, G., Petfalski, E., Lafontaine, D. L. J. and Tollervey, D. (2000). “Precursors to the U3 small nucleolar RNA lack small nucleolar RNP proteins but are stabilized by La binding.” Mol Cell Biol 20: 5415-5424.

Colley, A., Beggs, J. D., Tollervey, D. and Lafontaine, D.L.J. (2000). “Dhr1p, a putative DEAH-box RNA helicase, is associated with the box C+D snoRNP U3.” Mol Cell Biol 20: 7238-7246.


Massenet, S., Motorin, Y., Lafontaine, D.L.J., Hurt, E. C., Grosjean, H. and Branlant, C. (1999). “Pseudouridine mapping in the Saccharomyces cerevisiae spliceosomal U small nuclear RNAs (snRNAs) reveals that pseudouridine synthase pus1p exhibits a dual substrate specificity for U2 snRNA and tRNA.” Mol Cell Biol 19: 2142-2154.

Lafontaine, D.L.J. and Tollervey, D. (1999). “Nop58p is a common component of the box C+D snoRNPs that is required for snoRNA stability.” RNA 5: 455-467.

Fortes, P., Kufel, J., Fornerod, M., Polycarpou-Schwarz, M., Lafontaine, D.L.J., Tollervey, D. and Mattaj, I. W. (1999). “Genetic and physical interactions involving the yeast nuclear cap-binding complex.” Mol Cell Biol 19: 6543-6553.


Lafontaine, D.L.J., Bousquet-Antonelli, C., Henry, Y., Caizergues-Ferrer, M. and Tollervey, D. (1998). “The box H + ACA snoRNAs carry Cbf5p, the putative rRNA pseudouridine synthase.” Genes Dev 12: 527-537.

Lafontaine, D.L.J. and Tollervey, D. (1998). “Birth of the snoRNPs: the evolution of the modification-guide snoRNAs.” Trends Biochem Sci 23: 383-388.

Lafontaine, D.L.J., Preiss, T. and Tollervey, D. (1998). “Yeast 18S rRNA dimethylase Dim1p: a quality control mechanism in ribosome synthesis?” Mol Cell Biol 18: 2360-2370.

Lafontaine, D.L.J. and Tollervey, D. (1998). Regulatory aspects of rRNA modifications and pre-rRNA processing. Modification and editing of RNA. H. Grosjean and R. Benne. Washington, ASM Press: 281-288.


Housen, I., Demonte, D., Lafontaine, D.L.J. and Vandenhaute, J. (1997). “Cloning and characterization of the KlDIM1 gene from Kluyveromyces lactis encoding the m2(6)A dimethylase of the 18S rRNA.” Yeast 13: 777-781.


Lafontaine, D.L.J. and Tollervey, D. (1996). “One-step PCR mediated strategy for the construction of conditionally expressed and epitope tagged yeast proteins.” Nucleic Acids Res 24: 3469-3471.


Lafontaine, D.L.J., Vandenhaute, J. and Tollervey, D. (1995). “The 18S rRNA dimethylase Dim1p is required for pre-ribosomal RNA processing in yeast.” Genes Dev 9: 2470-2481.

Lafontaine, D.L.J. and Tollervey, D. (1995). “Trans-acting factors in yeast pre-rRNA and pre-snoRNA processing.” Biochem Cell Biol 73: 803-812.


Lafontaine, D.L.J., Delcour, J., Glasser, A. L., Desgres, J. and Vandenhaute, J. (1994). “The DIM1 gene responsible for the conserved m6(2)Am6(2)A dimethylation in the 3′-terminal loop of 18 S rRNA is essential in yeast.” J Mol Biol 241: 492-497.

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