Detecting Material State Changes in Condensates with DHM
Digital Holographic Microscopy (DHM) was applied to detect membraneless biomolecular condensates and membrane-bound organelles without staining. The nucleolar optical thickness was defined for characterizing the material state of the nucleolus. Ribosome biogenesis is initiated in the nucleolus, a multiphase biomolecular condensate formed by liquid-liquid phase separation. The nucleolus is a…